Generator

Part:BBa_K1343000:Design

Designed by: Shira Attias   Group: iGEM14_Technion-Israel   (2014-09-22)


P_tet->NheI->RBS->luxI->double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part has been sequenced and has been checked with a detector strain which detects AHL and produce color. We know for sure that it produces AHL.


Source

P_tet: BBa_I759002- we used only the promoter, without the rest of the part (spacer (including the RBS)-cro complex) and added few bp as a spacer. RBS: BBa_B0030. luxI: BBa_C0061 but we removed the LVA tag and added stop codon: "tag". Double terminator BBa_B0015

References